Larvicidal Activities of Entomopathogenic Nematode Steinernema carpocapsae and Zinc Oxide Nanoparticles Against the Black Cutworm, Agrotis ipsilon

El-Didamony, Samia E; Elshandidy, Nourhan M.; Negm, Heba Tallah E.; Mansour, Amany S.; El- Said, Rawan U.; Sabbah, Fayza M. E.; Adly, Alaa A.; Abdel Haliem, Walaa I. M.; Ghaly, Sawsan A.; Sakr, Alaa N.; Ahmed, Tasneem M. Ahmed M.; Tail, Marwa A. H.; Mansour, Fatma A.; Ismail, Esraa G.; Abdul Fattah, Karima A.; Ali, Reham S. S.; Ali, Asmaa A. B. Ali A. B.; Dawood, Eman A. G.; Menshawy, Esraa H.; Atwa, Wedad A.; Taha, Mostafa A.; Abass, Afaf Ab ELwahab; Zu- Elhemma, Asmaa; Mahmoud, Hanaa I.; Zayed, Abdelbaset B.; Elshaier, Manal Elsayed; Moselhy, Walaa A.; Kadada, Hala M.; Hamed, Fatma Z.; Elqady, Enayat M.; Salem, Hend H.; Gomaa, Eman El-Said; Mohammed, Shaimaa H.

doi:10.21608/ijtar.2025.203237.1053

Larvicidal Activities of Entomopathogenic Nematode Steinernema carpocapsae and Zinc Oxide Nanoparticles Against the Black Cutworm, Agrotis ipsilon

Document Type : Original Article

Authors

¹ Zoology and Entomology Department, Faculty of Science, Al-Azhar University

² Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Nasr City, Cairo-11884, Egypt

³ AL Azhar university-Faculty of science (Girls)-Zoology and Entomology Department

⁴ Zoology and Entomology Department , Faculty of Sciences , Al-Azhar University

10.21608/ijtar.2025.203237.1053

Abstract

The present study was conducted to determine the effect of entomopathogenic nematode Steinernema carpocapsae (S. carpocapsa) infective juveniles and zinc oxide nanoparticles (ZnO NPs) against Agrotis ipsilon larvae. Fourth larval instar was fed on castor leaves treated with different concentrations of S. carpocapsae (20, 40, and 80 IJs/larva) and ZnO NPs (150, 300, and 450ppm). Further biochemical assays and some histological studies were carried out to explain the effect of tested larvicides on A. ipsilon. The mortality percent of 4th larval instar due to the S. carpocapsae and ZnO NPs treatment increased up to 86.67 and 96.67%, respectively after 96 hours of application. The median lethal concentration (LC50) of S. carpocapsa IJs were 66.93, 38.71, and 25.86ppm at 48, 72, 96hrs PT, respectively. The corresponding LC50 values of ZnO NPs were 773.42, 396.70, 273.58, and 182.46ppm at 24, 48, 72, 96hrs PT, respectively. The results of biochemical assays and histological examination proved the lethal effects of the tested items.

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